akt1 protein kinase Search Results


akt1  (MedChemExpress)
93
MedChemExpress akt1
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Akt1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt  (Proteintech)
96
Proteintech p akt
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt  (Proteintech)
96
Proteintech akt
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p akt  (Proteintech)
96
Proteintech anti p akt
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Anti P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti total protein kinase b akt  (Rockland Immunochemicals)
90
Rockland Immunochemicals anti total protein kinase b akt
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Anti Total Protein Kinase B Akt, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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αser1177 phospho enos  (Rockland Immunochemicals)
93
Rockland Immunochemicals αser1177 phospho enos
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
αser1177 Phospho Enos, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotin  (Rockland Immunochemicals)
93
Rockland Immunochemicals biotin
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Biotin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti akt1  (Rockland Immunochemicals)
92
Rockland Immunochemicals anti akt1
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Anti Akt1, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho akt1 s129  (Boster Bio)
94
Boster Bio phospho akt1 s129
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Phospho Akt1 S129, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt antibody  (Boster Bio)
93
Boster Bio p akt antibody
Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
P Akt Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and AKT1 in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.

Journal: Science advances

Article Title: Three-dimensional histological electrophoresis enables fast automatic distinguishment of cancer margins and lymph node metastases.

doi: 10.1126/sciadv.adg2690

Figure Lengend Snippet: Fig. 2. Verification of the 3D histological electrophoresis distinguishing tumors from adjacent nonmalignant tissues. (A) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. (B) Western blotting for detecting overexpressed albumin and AKT1 in the 4T1 tumor sample. (C) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (**P < 0.05). (D) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. (E) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. (F) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. (G) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. (H) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis (n = 3 mice per group, ****P < 0.05). (I) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis (n = 3 mice per group). (J) NIR images and signal quantifi- cation of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. (K) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal differ- ence between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.

Article Snippet: AKT1 was purchased from MedChemExpress.

Techniques: Electrophoresis, Fluorescence, Labeling, SDS Page, Western Blot, Immunohistochemistry, Muscles